Murine Models of Bone and Brain Metastasis for Preclinical Cancer Research

Tumor metastasis is the primary driver of cancer mortality and therapy resistance, highlighting urgent need for clinically relevant preclinical models. GemPharmatech has developed robust murine models for bone and brain metastasis. Bone metastasis was established via intracardiac injection of MDA-MB-231-Luc cells, followed by in vivo selection of a highly bone-tropic subclone, confirmed by bioluminescence imaging and osteolytic lesions. Brain metastasis was induced through carotid artery injection of LLC1-Luc cells, enabling efficient tumor dissemination to the brain. These models provide platforms for studying metastatic mechanisms and evaluating novel therapies, advancing translational cancer research.