Preclinical Pathology Services

GemPharmatech's histopathology laboratory provides end-to-end tissue analysis services supporting drug discovery, preclinical safety assessment, and translational research. From tissue processing and embedding through sectioning, staining, and imaging, our team handles a broad range of tissue types — delivering reliable, reproducible morphological data at every stage of your program.

Advantages

Why Work with GemPharmatech?

A dedicated team of 10+ pathologists and 20 pathology technicians, processing over 60,000 slides and completing over 60,000 digital whole-slide scans annually — reflecting both the scale and consistency of our diagnostic capabilities.
Equipped with a comprehensive suite of professional Leica pathology equipment to meet high-throughput experimental demands.
High-throughput whole-slide scanning that enhances diagnostic efficiency and accuracy, while providing a reliable archive of slide images for long-term reference.
Beyond routine histopathological evaluation, our team provides full efficacy and toxicity assessments tailored to the specific requirements of your program.
Extensive experience in pathological examinations across various disease models ensures smooth project progression in all aspects.

Capabilities

What we offer

Our staining capabilities span H&E, IHC, ICC, IF, and specialized stains including PAS, Sirius Red, and Oil Red O, giving researchers the tools to characterize disease pathology, evaluate treatment effects, and meet the demands of regulatory studies. For projects requiring quantitative data, HALO AI-powered digital image analysis enables precise quantification of positive area, percent positive cell number, and spatial distribution.

All imaging and whole-slide scanning is reviewed and interpreted by experienced pathologists with extensive experience in preclinical safety and efficacy evaluation. Our pathology team consists of 10 pathologists, including three members certified by the Chinese Society of Veterinary Pathology.

Our services cover every stage of the tissue analysis workflow:

Necropsy and Tissue Collection
Tissue Embedding and Sectioning (Paraffin, Frozen)
Histochemical Staining (H&E, Special Staining)
Immunostaining: IHC, IF, ICC, Tyramide Signal Amplification (TSA)

Histopathology, IHC & Digital Pathology Services

Our pathology platform supports the full arc of preclinical tissue analysis, from initial necropsy through to AI-powered digital quantification. Equipped with professional-grade instrumentation from Leica and Olympus, we deliver precise, reproducible pathological data across a broad range of therapeutic areas — including oncology, cardiovascular, autoimmune, neuroscience, and metabolic disease — with disease-specific protocols.

Molecular Pathology: ISH, FISH, RNAScope
Histopathological Evaluation
HALO AI Digital Pathology & Quantitative Analysis

Tissue Collection and H&E Staining

We provide comprehensive tissue collection and processing services across a wide range of organs and tissues. All tissues are processed using standardized protocols for paraffin embedding, frozen sectioning, and H&E staining to ensure consistent, high-quality morphological assessment.

Our H&E staining service provides clear visualization of tissue morphology for routine pathological evaluation, supporting both standard screening and detailed diagnostic assessment across preclinical studies.

For example, in our neuroscience pipeline, we specialize in the dissection and processing of delicate neural and sensory tissues — such as whole brain, spinal cord, and eye globe — ensuring specimen integrity for accurate pathological evaluation across CNS, PNS, and sensory organ studies.

Special Staining Capabilities

Staining type	Application
Sirius Red	Used to distinguish different types of fiber types. The collagen fibers are red, the nuclei are green, and the rest are yellow.
Masson's trichrome	Mainly used to detect the degree of fibrosis in different tissues.
Safranine O-Fast Green	Reflects the structure of cartilage tissue.
TRAP (Tartrate-Resistant Acid Phosphatase)	Can directly identify and analyze the status of osteoclasts.
Oil Red O (ORO)	Presence of fat or lipids in tissue/section
Congo Red	ldentify the type of amyloid
Fontana-Masson	Can be used to identify melanin and melanin-producing cells
Periodic acid–Schiff (PAS)	Mainly used to detect sugars in tissues
Golgi Stain	Observe the overall picture of neurons in the central nervous system, especially the detailed morphology of their dendrites and axons.
Toluidine blue	Can be used for detection of mast cells
Wheat germ agglutinin (WGA)	It is most commonly used to stain cells of smooth muscle and striated muscle
Brilliant Cresyl Blue	Mainly used for in vivo staining of reticulocytes
Alcian	It is mainly used for locating and displaying goblet cells that secrete acidic mucus and the acidic mucin they secrete
Giemsa	Commonly used in blood diseases to observe inflammatory
TTC (2,3,5-triphenyltetrazolium chloride)	Commonly used indicators to evaluate cerebral ischemic injury
Timm copper deposition	It is mainly used for locating and displaying high concentrations of heavy metal ions in the tissue, especially zinc (Zn²⁺) and copper (Cu²⁺).

IHC&IF Staining

(1)IF & mIHC

(2)IHC

Histopathology, IHC & Digital Pathology Services

Case Studies

Case Studies & Validation Data

Histopathology, IHC & Digital Pathology Services

Multiplex immunofluorescence analysis via HALO AI.

The left panel coutains the original quintuple fluorescence image of the tumor, where two of the channels are displayed. The right panel demonstrates HALO's automated multi-marker phenotyping capability. HALO's classifier integrates signals from two fluorescent channels to assign each cell a unique phenotype identity, pseudocolored here for visualization.
WGA-stained cardiac tissue section.

HALO automatically segments individual cardiomyocyte boundaries (magenta outlines) for cell counting and morphometric analysis.(Green: WGA, Blue: DAPI).
Retinal Whole Mount Fluorescence Staining with HALO Vascular Area Quantification.

The left panel shows a retinal whole mount with fluorescence staining highlighting the vascular network in red. The right panel displays the HALO analysis output, where yellow masks mark the segmented vascular structures, enabling automated quantification of retinal vessel area percentage.
COL1A1 IHC on liver tissue with HALO quantification.

The left panel shows a COL1A1-stained liver section with brown DAB-positive signal and blue hematoxylin counterstain (100 μm scale bar). The right panel shows HALO analysis output: green marks COL1A1-positive areas and red marks nuclei, enabling automated quantification of COL1A1-positive area percentage.
Sirius Red staining of a NASH model liver section showing collagen deposition.

HALO analysis identifies green regions as fibrosis-positive areas (collagen) and red regions as fibrosis-negative tissue (parenchyma), with fibrosis-positive area percentage quantified.

Pathological Analysis of Disease Models

Study on the Efficacy of Anti-Mouse IL-4RA in a HDM-Induced Asthma Model in BALB/c Mice.
Pathology feature of Asthma Model:

PAS Positive (yellow arrow), bronchial epithelial hyperplasia (green arrow), alveolar wall thickening (blue arrow), single-nucleated cell (red arrow), neutrophil (black arrow). First row: Bar=200μm; Second row: Bar=50μm.
Efficacy Evaluation in a DSS-Induced HSC-NCG-M/hIL15 Immunocompromised Mouse Model of IBD.
Pathology feature of the IBD Model:
- IBD severity
- Hyperplasia
- Ulcers
- Lesion area
Ulcer (green arrow), mononuclear cells (red arrow), neutrophils (black arrow), fibrous connective tissue proliferation (yellow arrow), mucosal epithelial hyperplasia (purple arrow). First row: Bar=500 μm; Second row: Bar=100 μm.
Efficacy Testing of Anti-OX40L in an MC903-DNFB-Induced Dermatitis Model.
Pathology feature of Psoriasis Model:
- Inflammatory cell infiltration score
- Measurement of dermal thickness
- Measurement of epidermal thickness
Edema (gray arrow), epidermal thickening (blue arrow), hyperkeratosis (purple arrow), fibrous connective tissue proliferation (yellow arrow), mononuclear cells (dark red arrow), neutrophils (black arrow). First row: Bar=200 μm; second row: Bar=50 μm.