Alport Syndrome

Panel A Urine mALB and UACR (mALB/CREA) levels were markedly elevated in male Col4a3-KO mice, with levels rising progressively with age, indicative of impaired renal function.

Panel B Urine mALB and UACR (mALB/CREA) levels were markedly elevated in female Col4a3-KO mice, with levels rising progressively with age, indicative of impaired renal function.

Panel C Peripheral blood analysis revealed that 8-week-old male B6-Col4a3-KO mice displayed significant reductions in RBC (red blood cell), HGB (hemoglobin), RETIC-ABS (absolute reticulocyte count), and HCT (hematocrit), confirming an anemic phenotype.

Panel D Mice were treated with Ramipril, an ACE-inhibitor. Treatment was initiated in male mice at 4 weeks of age. Five weeks post-treatment, UACR levels were significantly lowered following ramipril intervention.

Statistical analysis was performed using one‑way ANOVA, and all data is presented as mean ± SEM. *p<0.05, **p<0.01, ***p<0.001,****p<0.0001.