Custom Breeding and Colony Management

Suitable  for strains with good fertility and when natural mating background is preferred

Natural breeding provides a reliable and physiologically relevant approach for colony expansion. It maintains normal reproductive processes, reducing the risk of stress or technical variability introduced by assisted reproductive technologies. This approach helps preserve genetic stability and supports the generation of healthy offspring with consistent phenotypes. Natural breeding is cost-effective and well-suited for long-term colony maintenance, while still allowing for scalable production when properly managed.

Timeline: One generation of mouse breeding typically takes approximately 3 months.

Key Advantage: Natural breeding maintains genetic stability and supports the generation of healthy offspring with consistent phenotypes. Cost-effective for long-term colony maintenance.

In Vitro Fertilization with  Embryo Transfer

Ideal for limited animal availability, poor fertility, or complex genetic models

This approach offers higher efficiency with fewer input animals required compared to natural breeding methods.

Input requirements:

• Males: 2–3 (3–6 months old, healthy, correct genotype)

• Females: ≥6 (preferably 3–4 weeks or 7–8 weeks old)

Technical Method: In Vitro Fertilization (IVF) + Embryo Transfer (ET)

Workflow:

1. Original breeder animal validation and preprocessing (health check & genotyping)

2. Superovulation of donor females using proprietary Sova superovulation reagents

3. IVF and embryo culture to 2-cell stage

4. Embryo transfer into SPF pseudopregnant recipients

5. Postnatal care, weaning, and genotyping

Timeline: ~7–9 weeks from original breeder animal validation to delivery of weaned, genotyped mice

Delivery: SPF offspring at 3–4 weeks old (typically ≥3 genotype-validated mice)

Key Advantage: Highly synchronized cohorts delivered in a single batch, enabling large-scale 

synchronized production and age-matched cohorts for pharmacology studies and model supply.

Comprehensive technology coverage to overcome breeding barriers

With a well-established and versatile technical system, we support breeding needs across different sexes, ages, and challenging genetic backgrounds. Even for difficult strains, subfertile males, or lethal/semi-lethal genotypes, we can reliably produce healthy offspring. By combining assisted reproduction with cryopreservation technologies, we offer a synergistic solution that safeguards valuable genetic resources and eliminates the risk of colony loss.

Technical Method: We offer a full suite of reproductive technologies, including IVF, ICSI, embryo transfer, ovarian transplantation, and sperm/embryo cryopreservation.

Applications:

• Difficult breeding strains

• Low fertility or reproductive failure

• Rapid colony expansion

• Genetic resource preservation

• Rederivation support

Workflow:

1. Project consultation and breeding strategy design (IVF, ICSI, sperm revival, etc.)

2. Animal provision and health check

3. Procedure execution and monitoring

4. Delivery of SPF, genotyped offspring

Typical timeline: ~3–4 months (standard IVF + ET workflow)

Solutions for Common Breeding Issues:

Note: Optimal breeding age varies by strain; generally, males perform best at 3–6 months and females at 8 weeks–4 months.

High-throughput, large-scale production

Two-cell stage mouse embryo

Our industry-leading reproductive technologies consistently deliver exceptional fertilization and litter rates, ensuring reliable, reproducible model production at every scale.

Key Advantages:

• High-throughput, large-scale production capable of generating thousands of age-matched mice in a single batch, supported by extensive recipient animal resources, state-of-the-art equipment, and a highly skilled technical team.

• Flexible, tailored breeding strategies developed around the unique physiological characteristics of each strain, drawing on years of hands-on mouse breeding expertise.

• Proprietary Sova superovulation reagents significantly improve ovulation efficiency while aligning with 3R principles — reducing the number of animals needed without compromising output.

• Independently developed and manufactured core reagents ensure a stable, secure supply chain, free from the delays and variability.

Technical Method: In Vitro Fertilization (IVF) + Embryo Transfer (ET)

Workflow:

5. Original breeder animal validation and preprocessing (health check & genotyping)

6. Superovulation of donor females using proprietary Sova superovulation reagents

7. IVF and embryo culture to 2-cell stage

8. Embryo transfer into SPF pseudopregnant recipients

9. Postnatal care, weaning, and genotyping

Timeline: ~7–9 weeks from original breeder animal validation to delivery of weaned, genotyped mice

Key Advantage: Highly synchronized cohorts delivered in a single batch, enabling large-scale synchronized production for pharmacology studies, model supply, and large experiments.

Stringent facility management for contamination-free workflows

Our rederivation service upgrades mouse colonies from non-SPF to fully certified SPF status — systematically eliminating bacterial, viral, and parasitic pathogens to restore and guarantee the health status your research requires. Achieving that standard is based on a rigorously controlled environment at every stage of the process. Strict facility zoning, dedicated personnel workflows, and independent bio-clean rooms work in together to eliminate cross-contamination risks, ensuring that every animal that moves through our facility does so within a controlled and contamination-free environment.

100% rederivation success  — a proven track record of delivering clean, health-screened animals that meet the standards your research demands.

Key Advantages:

Proven 100% rederivation success rate — a track record of delivering clean, health-screened animals that meet the standards your research demands.

Dual-species expertise — mature IVF and embryo transfer systems for both mouse and rat models, backed by extensive hands-on experience across both species.

Full traceability and documentation — standardized procedures with complete end-to-end records, supporting research validation and regulatory requirements.

Optimized recipient management — tailored approaches including cesarean section and foster nursing to maximize survival rates and ensure successful colony establishment.

Strict Operational Workflows: We implement strict zoning and personnel management with dedicated workflows and independent bio-clean rooms. Our process ensures efficient and contamination-free operations from model receipt to final delivery.