huHSC-NCG

Autoimmune Disease Models
Autoimmune Nephropathy ModelsAsthma ModelsSystemic Lupus Erythematosus (SLE) ModelsSjögren's Syndrome ModelsAutoimmune Dermatoses Models Neuroimmune Disease ModelsInflammatory Bowel Disease ModelsRheumatoid Arthritis ModelsImmune Reconstitution Autoimmune Disease Models
Metabolic Disease Mouse Models
Obesity ModelsDiabetes ModelsMASH ModelsOsteoporosis ModelsHyperuricemia Models
Wild Mouse 750
Wild Mouse 750
Rare Disease Mouse Models
Autosomal Dominant Polycystic Kidney Disease (ADPKD)Progressive Muscular Dystrophy ModelsHemophilia A Models
Neurological Disease Mouse Models
Alzheimer's Disease (AD) ModelsHumanized Alzheimer's Disease (AD) ModelsParkinson's Disease (PD) ModelsHuntington's Disease (HD) ModelsAmyotrophic Lateral Sclerosis (ALS) ModelsTransthyretin Amyloidosis (ATTR) ModelsPain Mouse ModelsDuchenne Muscular Dystrophy (DMD) Models
Oncology Mouse Models
Human Xenograft ModelsDrug ResistanceT-Cell EngagersHumanized Immune Checkpoint ModelsHumanized Immune System ModelsSpontaneous Tumor Models
Cardiovascular Disease Mouse Models
Heart Failure ModelsHypertension ModelsAtherosclerosis ModelsAdvanced Cardiovascular and Metabolic Complication ModelsHyperlipidemia ModelsHypertropic Cardiomyopathy ModelsStroke ModelsThrombosis Models
Humanized Immune System Models
huPBMC-NCGhuHSC-NCG-XhuHSC-NCG-hIL15huHSC-NCG
Organs or Tissue Humanized Mouse Models
Wild Mouse 765
Wild Mouse 765

huHSC-NCG mice are generated by implanting Hematopoietic Stem Cells (CD34+HSC) into GemPharmatech's severe immunodeficient NCG mice. The transplanted HSC in irradiated NCG mice then differentiates into a variety of hematopoietic or immune cells, including T cells, B cells, and NK cells. With a long survival cycle and stable reconstituted human immune system, this model can be used for long-term in vivo studies for testing drug efficacy, making it an ideal model for preclinical drug evaluation.


Strategy

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Applications

  • Preclinical anti-tumor efficacy study and safety evaluation

  • Testing of immuno-oncology therapies


Supporting data

1. Detection of the marrow clearing effect in NCG mice

    Without irradiation                                With irradiation

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Irradiation can eliminate the hematopoietic stem cells in the bone marrow, thereby improving the level of reconstruction after transplantation of human HSC. The results showed that the proportion of mCD45+mCD117+ cell population after irradiation was 1.11%, which was significantly lower than the non-irradiated group (13.5%). This result suggests that 150 cGys dose irradiation of 6-7 weeks NCG mice can achieve the desired marrow clearing effect.


2. The survival curve and the body weight change of the huHSC-NCG

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The huHSC-NCG mice can survive more than 37 weeks. In the first period, the body weight of huHSC-NCG mice tended to increase and was not significantly different from that of non-irradiated NCG mice that did not undergo HSC reconstitution. Over time, the body weight of huHSC-NCG mice decreased. By the end of the experiment, it was significantly lower than that of NCG mice that had not undergone irradiation and HSC reconstitution (***, P < 0.001). Overall, the body weight of huHSC-NCG mice increased over time without significant weight loss during long-term immune reconstitution, making this a good model for long-term investigations. Data are shown as mean±SEM.


3. The human immune reconstitution level of the huHSC-NCG mice

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The human immune cell levels in peripheral blood of huHSC-NCG mice were detected by flow cytometry. These are primarily T cells, many immature B cells, and a modest number of NK cells. 


4. The reconstitution levels of humanized immune cells in the huHSC-NCG mice at week 16

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Flow cytometry of huHSC-NCG mice peripheral blood at week 16 revealed that a range of human-derived immune cells could be recognized, with the  proportion of human leukocytes at more than 20%, indicating that the humanized model of huHSC-NCG was successfully established in this experiment.


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