Tissue Sample Analysis
Thymus: Research on T-cell differentiation and development.
Lymph nodes: Analysis of T-cells, B-cells, and DC cells.
Bone marrow: Research on B cell development, red blood cell development, and hematopoietic stem cells.
Kidney: Analysis of inflammatory cell infiltration.
Lung: Analysis of immune cell infiltration in mouse lung tissue in tumors or inflammatory conditions.
Skin: Analysis of cellular inflammatory infiltration.
Brain: Analysis of the infiltration of inflammatory cells within the organization.
Experimental Case Study 1: Thymus T cell development.
Population | Gating Step | ||||
Th Cells | Live single | mCD4+ | mCD8- | / | / |
Tc Cells | Live single | mCD4- | mCD8+ | / | / |
DP Cells | Live single | mCD4+ | mCD8+ | / | / |
DN Cells | Live single | mCD4- | mCD8- | / | / |
DN2 Cells | Live single | mCD4- | mCD8- | mCD44+ | mCD25+ |
DN3 Cells | Live single | mCD4- | mCD8- | mCD44- | mCD25+ |
DN1 Cells | Live single | mCD4- | mCD8- | mCD44+ | mCD25- |
DN4 Cells | Live single | mCD4- | mCD8- | mCD44- | mCD25- |
Experimental Case Study 2: Analysis of Renal immune cell (12-color panel)
Population | Gating Step | ||||
Total Leukocytes | Live single | mCD45+ | / | / | / |
Neutrophils | Live single | mCD45+ | mCD11b+ | Ly6G+ | / |
Monocytes | Live single | Not Neutrophils | mCD11b+ | Ly6C hi | / |
Eosinophils | Live single | Not monocytes | mCD11b+ | SSC-H hi | / |
Macrophage | Live single | Not eosinophils | mCD11b+ | F4/80+ | / |
DC Cells | Live single | Not eosinophils | mCD11b+ | mCD11c+ | / |
T Cells | Live single | Not eosinophils | mCD3+ | mCD335- | / |
B Cells | Live single | Not eosinophils | mCD19+ | / | / |
NK Cells | Live single | Not eosinophils | mCD3- | mCD335+ | / |
Th Cells | Live single | Not eosinophils | mCD3+ | mCD4+ | mCD8- |
Tc Cells | Live single | Not eosinophils | mCD3+ | mCD4- | mCD8+ |
Experimental Case Study 3: Analysis of infiltrating immune cells in brain tissue (10-color panel)
Population | Gating step | ||||
Total Leukocytes | Live single | mCD45+ | / | / | / |
Neutrophils | Live single | mCD45+ | mCD11b+ | Ly6G+ | / |
Monocytes | Live single | Not Neutrophils | mCD11b+ | Ly6C hi | / |
Eosinophils | Live single | Not monocytes | mCD11b+ | SSC-H hi | / |
Macrophage | Live single | Not eosinophils | mCD11b+ | F4/80+ | / |
T cells | Live single | Not eosinophils | mCD3+ | mCD19- | / |
B cells | Live single | Not eosinophils | mCD19+ | mCD3- | / |
Th Cells | Live single | Not eosinophils | mCD3+ | mCD4+ | mCD8- |
Tc Cells | Live single | Not eosinophils | mCD3+ | mCD4- | mCD8+ |
Advantages
Extensive experience processing different tissues.