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CRISPR/Cas9 Technology

CRISPR/Cas9 Technology

CRISPR/Cas9 is the most common gene editing technology currently which can be used to establish gene-edited mice including CKO/KO/KI/PM.


Advantages

1.High quality: Executing the most stringent quality control standards, Realtime PCR detection, 0 random insertion, 0 serial recombination

2.High efficiency: GPTech optimized efficient gene editing platform, higher success rate and shorter delivery cycle

3.Multi-modification: multiple loci or multiple genes of the target gene were knocked out at the same time, and the ultra-long target fragment 23Mbps was successfully KO

4.Multi-background: C57BL/6J, C57BL/6N, BALB/C, FVB, FVB, NOD/LTJ, NCG, etc.


CRISPR/Cas9 is a gene editing technology that achieves sprcific gene modification by cleavage of target gene loci by Cas9/sgRNA complexes. Compared with other technologies (TALEN, ZFN, ES technology), it is simple, fast, and low cost. Now, GPTech constructs more than 4000 mouse models per year through CRISPR/Cas9 technology.The construction capacity ranks among the best in the world. Details of making KO, CKO, KI, and PM gene-edited mice by CRISPR/Cas9 technology are as follows:


Gene knockout(Cas9 KO)

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CRISPR/Cas9 KO Strategy

Conditional gene knockout(Cas9 CKO) 

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CRISPR/Cas9 KO Strategy

Gene knockin(Cas9-KI)

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CRISPR/Cas9 KI Strategy 

点突变(Cas9-PM)

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CRISPR/Cas9 PM Strategy


Time:2019-11-22