Check-point humanized mouse models
The immune checkpoint factor are a series of transmembrane proteins of the immune cells or tumor cells. These proteins could transmit the immune activation or inhibition signal, which regulate the behavior and activity of the immune cells by binding with a specific receptor or ligand. Immuno-Checkpoint antibodies binding to check-point receptor/ligand could specificly block or activate the immune-related signaling transduction, so that immune cells can be activated even in tumors, thereby effectively removing tumor cells.
Ideal animal models are needed to evaluate the efficacy and safety of immune checkpoint antibodies. On one hand, the work of immune checkpoint antibodies require a complete immune system, on the other hand, different species have different conserved immune checkpoint proteins and different antibody recognition sites. Thus the efficacy and safety evaluation of immune checkpoint humanized mice have higher clinical conversion rate.
BALB/c is an inbred mouse line which is widely used in immunology and oncology. Many homologous tumor cell lines are derived from BALB/c background, such as colon cancer cell line CT26, breast cancer cell line 4T1, liver cancer cell line H22 and B cell carcinoma cell line A20, and so on.
C57BL/6 is the most widely circulated mouse strain, and has been extensively used in genetics, oncologic and other studies. The homologous tumor cell lines derived from C57BL/6 background are colon cancer cell line MC38, melanoma cell line B16F10, and so on.
BALB/c and C57BL/6 backgrounds have different immune characteristics, such as the different expression levels of CD14 and toll-like receptor gene in the two backgrounds. The response to some viral infections also varies. All these differences of genetic background and cell lines will result in different sensitivities to response to the same antibody.
1. Two background(BALB/c and C57BL/6) of immune checkpoint humanized mice
2. More than 40 mouse tumor cell lines for anti-tumor drug screening.
Targets (BALB/c and C57BL/6) :
Mouse derived cell lines