In 2012, we have joined the International Mouse Phenotype Consortium (IMPC), and completed the construction of an internationally standardized phenotypic analysis platform in 2013. The standardized analysis process begins at 9.5 days in the embryonic period of mice and ends at 16 weeks in the adult period of mice, covering 20 major tests including cardiovascular, facial features, energy metabolism, bone, neural behavior, blood biochemistry, blood cells, and tissue morphology for phenotypic screening of mice. As the only member of IMPC in China, we had completed the analysis of 250 strains and uploaded the research results to the DCC platform for data sharing. At present, based on the platform of phenotypic analysis, a phenotypic analysis service system including tumor, metabolism, overall phenotypic screening, and pharmacology and pathology has been established in combination with the self-developed new models of severely immunodeficient mice, such as NCG and immunocheckpoint humanized mice.
Metabolic cage experiment（Indirect Calorimetry）
Principle：After using standard gas calibration instrument accuracy of gas detection, feeding the mice in the metabolic cages, avoided human disturbance, by measuring the change gases (oxygen and carbon dioxide) in mice breathing entropy calculation, heat production, real-time detect changes of food intake in mice, and real-time activity in mice by infrared through frequency calculation.
Instruments：Oxymax indirect calorimetry system (Oxymax, Columbus Instruments)
Sample requirements：No more than 16 at a time
Period：Adapt to 24 hours，Continuously test for 48-72 hours
BW: body weight, at the beginning and end of the experiment; VO2: oxygen consumption; VCO2: production of carbon dioxide; RER: respiratory exchange rate, RER=VCO2/VO2; HEAT: calorific value, calculating the HEAT generated by the consumption of oxygen; FEED: food intake; Total activity; Ambulatory activity: a static activity.
Mouse bone density and body fat ratio (DEXA)
Principle：Bone mineral content and density were measured, and body composition (fat and lean meat content) of mice were analyzed using Dual Energy X-ray Absorptiometry.
Instrument：Bone mineral density meter (PIXImus 2)
Samples：Mice (internal mice, in vitro tissue, testing at least 4)
AAALAC-certified first-class animal facility
Diverse functional analysis services based on IMPC platform
One-stop mouse experiment platform