Drug efficacy on Alzheimer's disease model
Introduction：Alzheimer's disease (AD) is the most common cause of dementia among elderly population worldwide. AD is mainly characterized by the accumulation and aggregation of amyloid-beta (Aβ) peptides, which form over time senile plaques in the brain parenchyma and microaggregates in the cerebral vasculature. APPswe mice, as a commonly used model of Alzheimer's disease, were used to test the efficacy of drugs for Alzheimer's disease.
Strain：B6C3-Tg(APPswerPSEN1dE9) /Nju mouse
Group Size：5-10 mice per group，3-8 groups
Grouping：Negative control, Vehicle control, Positive drug control，Test drug (low , middle and high dose level).
Period: 8 weeks
Inspection Items: Behavior tests(Morris water maze,Y maze, etc.), Pathological tests (immunohistochemistry, special staining, etc.), protein immunization, qRT-PCR, etc.
At the same time, other chemically induced Alzheimer's disease models were also performed: the localised injection of various toxins in brain's regions, and subacute senescence model induced by intra-abdominal injection of d-galactose.
Immunohistochemistry showed the deposition of β-amyloid in the hippocampus of the brain tissue of APPswe at 10 months old