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SNP detection in mice and rats

Service introduction

SNP is single nucleotide polymorphisms. It is usually used for annual monitoring of genetic background of rats and mice to detect whether genetic mutation or genetic contamination occurs in them, and to test all kinds of mutant mice in batches.


Advantage

One-stop service: from primer design to result issuing, one-stop service that can be provided.

Rich experience: The total number of detection data points has reached more than 100,000 since the establishment of the high-throughput detection platform,

Stable genetic detection panel: SNP panel for genetic detection of 13 inbred mice and background detection of fast backcross mice was successfully developed.


Detail Introduction

SNP, that is single nucleotide polymorphism, mainly refers to nucleic acid sequence polymorphism caused by single nucleotide change with variation frequency greater than 1% in the population, including single-base conversion, inversion, insertion and deletion. As the third generation of genetic markers, SNP marker technology than other genetic markers are widely distributed, genetic stability is good, representative, second-class genotype, easier to achieve high throughput and automation monitoring genetic quality monitoring advantages, and is more and more used in agriculture, medicine and life science research field.

Our company uses KASP technology to realize accurate detection of SNP, which can satisfy you with the following requirements:

1. Annual monitoring of the genetic background of rats and mice: the annual genetic test of experimental animals is one of the essential testing measures to assess and ensure the quality of experimental animals, especially the core group, so it is necessary to monitor the genetic background regularly.

2. To detect whether there are genetic mutations or genetic contamination in rats and mice: if a new phenotype appears during the experiment, or it is not sure whether the mouse is mismatched.

3. Batch detection of various mutant mice can achieve accurate InDels typing classification (insertion and deletion).

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The classification diagram of genetic monitoring sites of BALB/cJ background lines with KASP technique (ex.)

The high-throughput competitive Allele Specific PCR (Kompetitive Allele Specific PCR,KASP) based on well-known SNP site information, based on high sensitivity fluorescence detection, can perform accurate bi-allele typing for Specific SNPs and InDels of various genome samples (including complex genomic DNA samples). The principle of this technique is similar to Taqman detection, with the accuracy consistent with the gold standard Taqman. Different from the traditional Taqman technique, it does not need to synthesize specific probe primers for each target. Its unique ARM PCR principle that allows all site detection to eventually be amplified using universal fluorescent primers. The optimized PCR system can meet the needs of high-throughput reactions at different sites, which not only has the accuracy of gold standard, but also reduces the experimental cost, and has better site adaptability than Taqman.

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The classification diagram of genetic monitoring sites of C57BL/6J background lines with KASP technique (ex.)

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The classification diagram of genetic monitoring sites of BALB/cJ background lines with KASP technique (ex.)


Operating Process

1d: Sample receipt and confirmation of site information

2ds: DNA extraction

1~5ds: primer design

3~15ds: Primer order

15ds: primer test

16~20ds: Replace the failed site

25~30ds: Experiment (including experiment, review, report provided, etc.)

Time:2019-11-19