Key words :

Mouse Mall
+1 (888) 899-5899
Online Inquiry

hPDL1 mice


BALB/c-hPDL1

Strain Name: BALB/cJGpt-Pdl1tm1(hPDL1)/Gpt

Strain Type: Knock-in

Strain ID: T003260

Background: BALB/cJGpt


Description

Programmed death-ligand 1(PDL1), also known as cluster of difffferentiation 274 (CD274) or B7 homolog 1(B7H1). A large number of studies have confirmed that,  in the tumor microenvironment, the expression of PDL1 on the surface of tumor cells is increased, and it binds to PD1 on activated T cellsto transmit negative regulatory signals, leading to apoptosis or immune disability of tumor antigen -specifific T cells, thereby suppressing immune response, inducing the escape of tumor cells.Blocking PD1/PDL1 signaling pathway with antibodies has become a classic method for tumor immunotherapy. The murine PDL1 gene was replaced

with its human counterpart to develop the BALB/c-hPDL1 mouse model. BALB/c-hPDL1 mice are the ideal animal model to evaluate the efficacy of human PD-L1 antibody.


Strategy

Strategy of humanized hPDL1 mice 

1.png


Application

1. Efficacy evaluation of human PDL1 inhibitors

2. Safety evaluation of human PDL1 inhibitors

3. Anticancer Drug Research and Development

4. Research on autoimmune diseases

Detection of PDL1 expression in BALB/c-hPDL1 mice

2.png

BALB/c-hPDL1 mice successfully express hPDL1 on the surface of T cells

T/B/NK cell ratio assay

3.png

There was no difference of T/B/NK cells proportion in wild-type, heterozygous and homozygous mice.

 

C57BL/6-hPDL1


Strain Name: B6/JGpt-Pdl1tm1(hPDL1)/Gpt

Strain Type: Knock-in

Strain ID: T003455

Background: C57BL/6NGpt


Description

Programmed death-ligand 1(PDL1),also known as cluster of difffferentiation 274 (CD274) or B7 homolog 1(B7H1). A large number of studies have confifirmed that,  in the tumor microenvironment, the expression of PDL1 on the surface of tumor cells is increased in the tumor microenvironment, and it binds to PD1 on activated T cells, to transmitting negative regulatory signals, leading to apoptosis or immune disability of tumor antigen - specifific T cells, thereby suppressing immune response, inducing the escape of tumor cells. Blocking PD1/PDL1 signaling pathway with antibodies has become a classic method for tumor immunotherapy. The murine PDL1 gene was replaced

with its human counterpart to develop the C57BL/6-hPDL1 mouse model. C57BL/6-hPDL1 mice the ideal animal model to evaluate the efficacy of human PD-L1 antibody.


Strategy

Strategy of humanized hPDL1 mice

4.png

 

Application

1. Efficacy evaluation of human PDL1 inhibitors

2. Safety evaluation of human PDL1 inhibitors

3. Anticancer Drug Research and Development

4. Research on autoimmune diseases

Detection of PDL1 expression in B6-hPDL1 mice

5.png

hPDL1 homozygous mice expressed human PDL1 on both T and B cell surfaces.

T/B/NK cell ratio assay

6.png

The proportion of T/B/NK cells in B6-hPDL1 homozygous, heterozygous and wild-type mice is similar.

In vivo efficacy test

TECENTRIQTM Atezolizumab)efficacy test(humanized murine colon cancer cell line MC38-hPDL1)

Tumor volume changes of mice in different groups

7.png

Body weight changes of mice in different groups

8.png

Significant tumor inhibition observed on homologous B6-hPDL1 mice bearing murine colon carcinoma MC38 tumor after the TECENTRIQTM treatment

Time:2019-12-10