Liver fibrosis model
Introduction：Carbon tetrachloride (CCl4) is the most widely used toxin to induce liver fibrosis. CCl4 induced mouse liver fibrosis model is suitable to test the effect of the anti-fibrosis drugs.
Strain C57BL/6J mouse and other gene editing mouse
Group Size：8-10 mice per group，5-8 groups
Grouping：Negative control, Vehicle control, Positive drug control，Test drug (high, middle and low dose level).
Period: 8-10 weeks
Inspection Items：Fibrosis scoring, Fibrosis marker assessment (TGFβ1、COL1A1、TIMP1、ACTA2 etc.), Histologic analysis (H&E staining, Sirius red staining)
1. Sirius red staining of the liver from control and CCl4 injected mice
Sirius Red staining of liver sections showed significant fibrosis in the liver of the CCl4-injected mice.